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Providers / Bioss Polyclonal Antibodies / Anti-Histone H1.4(Thr18) (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-3171R-A594

Price: 516 EUR

Get for 516 EUR on Gentaur.com

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Product details

Gene ID

3008

Modification site

Thr18

Swiss Prot

P10412

Subcellular locations

Nucleus

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Modification

Phosphorylation

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Target Protein/Peptide

Histone H1.4 Thr18

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Other name

Anti-Histone H1.4(Thr18) Polyclonal

Also known as

Histone H1.4(Thr18) Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Synonyms

H1E; H1.4; H1F4; H1s-4; dJ221C16.5; Histone H1.4; Histone H1b; Histone H1s-4; HIST1H1E

Antigen Source

KLH conjugated synthetic phosphopeptide derived from human Histone H1.4 around the phosphorylation site of Thr18

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Specificity

This phosphorylation site is found in Rat H1C as well, but not Human or Mouse H1C. This phosphorylation site is homologous to Thr18 in Rat H1C/H1D and Mouse H1D. Due to the highly conserved nature of these histones, this antibody may react with these members when phosphorylated at this site.Histone H1.4(Thr18)

Background information

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.