Product details

Subcellular locations

N/A

Swiss Prot

N/A

Target Protein/Peptide

ID3

Gene ID

3399

Modification site

None

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Other name

Anti-ID3 Polyclonal

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Also known as

ID3 Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with ID3

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human ID3

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Background information

ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Synonyms

1R21; bHLHb25; Class B basic helix-loop-helix protein 25; DNA binding protein inhibitor ID 3; DNA-binding protein inhibitor ID-3; HEIR 1; HEIR1; Helix loop helix protein HEIR 1; Helix loop helix protein HEIR1; Helix-loop-helix protein HEIR-1; HLH642; ID 3; ID like protein inhibitor HLH 1R21; ID like protein inhibitor HLH 462; ID-like protein inhibitor HLH 1R21; ID3; ID3_HUMAN; Inhibitor of DNA binding 3; Inhibitor of DNA binding 3 dominant negative helix loop helix protein.

Anti-ID3 (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-6229R-A594

Price: 489 EUR