Description:

    Size: 100 microliters

    Catalog no.: GENTObs-4094R-A594

    Price: 489 EUR

    Product details

    Gene ID

    994

    Modification site

    None

    Swiss Prot

    P30305

    Target Protein/Peptide

    Cdc25B

    Subcellular locations

    Cytoplasm

    Applications

    IF(IHC-P)

    Excitation emission

    590nm/617nm

    Concentration

    1ug per 1ul

    Conjugated with

    ALEXA FLUOR® 594

    Conjugated

    Alexa conjugate 1

    Applications with corresponding dilutions

    IF(IHC-P)(1:50-200)

    Clonality

    Polyclonal Antibody

    Clone

    Polyclonal Antibodies

    Other name

    Anti-Cdc25B Polyclonal

    Purification method

    Purified by Protein A.

    Group

    Polyclonals and antibodies

    Also known as

    Cdc25B Polyclonal Antibody

    Type

    Conjugated Primary Antibody

    Conjugation

    Alexa Fluor,ALEXA FLUOR® 594

    Host organism

    Rabbit (Oryctolagus cuniculus)

    Properties

    For facs or microscopy Alexa 1 conjugate.

    Specificity

    This antibody reacts specifically with Cdc25B

    Modification

    No modification has been applied to this antibody

    Antigen Source

    KLH conjugated synthetic peptide derived from human Cdc25B

    Cross reactive species

    Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

    Synonyms

    M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; CDC25B; CDC25HU2

    Storage

    Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

    Description

    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

    About

    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

    Cross Reactive Species details

    No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

    Background information

    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

    Advisory

    Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.