Product details

Target Protein/Peptide

CD34

Modification site

None

Gene ID

12490

Swiss Prot

Q64314

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Subcellular locations

Extracellular

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Clonality

Polyclonal Antibody

Other name

Anti-CD34 Polyclonal

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Applications

FCM, IF(IHC-P), IF(ICC)

Also known as

CD34 Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Specificity

This antibody reacts specifically with CD34

Modification

No modification has been applied to this antibody

Applications with corresponding dilutions

FCM(1:20-100), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Synonyms

AU496; Hematopoietic progenitor cell antigen CD34; CD34

Antigen Source

KLH conjugated synthetic peptide derived from mouse CD34

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus), Rabbit

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Properties

For facs or microscopy Alexa 1 conjugate.Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins (By similarity).

Anti-CD34 (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-2038R-A594

Price: 489 EUR