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Providers / Bioss Polyclonal Antibodies / Anti-NCF1/p47 phox(Ser359), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-11445R-A594

Price: 516 EUR

Get for 516 EUR on Gentaur.com

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Product details

Subcellular locations

N/A

Swiss Prot

N/A

Modification site

Ser359

Gene ID

653361

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Modification

Phosphorylation

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Target Protein/Peptide

NCF1/p47 phox Ser359

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Other name

Anti-NCF1/p47 phox(Ser359)

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Also known as

NCF1/p47 phox(Ser359) Antibody

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Cross reactive species

Human (Homo sapiens), Rat (Rattus norvegicus)

Specificity

This antibody reacts specifically with NCF1/p47 phox(Ser359)

Antigen Source

KLH conjugated synthetic phosphopeptide derived from human NCF1 around the phosphorylation site of Ser359

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Synonyms

NCF1Ser359; p47 phoxSer359; NCF1 phospho S359; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF 47K; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox organizing protein 2; NOXO2; p47 phox; p47phox; ;SH3 and PX domain containing protein 1A; SH3PXD1A; NADPH oxidase p47 phox; NCF1_HUMAN.

Background information

The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.