Description:

    Size: 100 microliters

    Catalog no.: GENTObs-5300R-A594

    Price: 516 EUR

    Product details

    Subcellular locations

    N/A

    Swiss Prot

    N/A

    Gene ID

    23586

    Modification site

    Thr170

    Applications

    IF(IHC-P)

    Concentration

    1ug per 1ul

    Excitation emission

    590nm/617nm

    Target Protein/Peptide

    DDX58 Thr170

    Modification

    Phosphorylation

    Conjugated with

    ALEXA FLUOR® 594

    Conjugated

    Alexa conjugate 1

    Clonality

    Polyclonal Antibody

    Applications with corresponding dilutions

    IF(IHC-P)(1:50-200)

    Clone

    Polyclonal Antibodies

    Purification method

    Purified by Protein A.

    Group

    Polyclonals and antibodies

    Type

    Conjugated Primary Antibody

    Conjugation

    Alexa Fluor,ALEXA FLUOR® 594

    Other name

    Anti-DDX58 (Thr170) Polyclonal

    Host organism

    Rabbit (Oryctolagus cuniculus)

    Also known as

    DDX58 (Thr170) Polyclonal Antibody

    Properties

    For facs or microscopy Alexa 1 conjugate.

    Cross reactive species

    Human (Homo sapiens), Mouse (Mus musculus)

    Specificity

    This antibody reacts specifically with DDX58 (Thr170)

    Antigen Source

    KLH conjugated synthetic phosphopeptide derived from human DDX58 around the phosphorylation site of Thr170

    Storage

    Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

    Description

    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

    About

    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

    Cross Reactive Species details

    No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

    Synonyms

    DEAD/H Asp-Glu-Ala-Asp/His box polypeptide RIG-I; DKFZp434J1111; DKFZp686N19181; FLJ13599; C330021E21; OTTHUMP00000045225; DEAD Asp-Glu-Ala-Asp box polypeptide 58; DEAD Asp Glu Ala Asp/His box polypeptide; DEAD box protein 58; Probable ATP dependent RNA helicase DDX58; Retinoic acid inducible gene 1 protein; RIG I; rig-I; RIG1; rigi; RNA helicase.

    Advisory

    Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

    Background information

    The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG1/DDX58) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG1/DDX58 activates the kinases TBK1 and IKKe through the adaptor protein IPS1. These kinases then phosphorylate the transcription factors IRF3 and IRF7 which are essential for the expression of type-I interferons. RIG1/DDX58 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.