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Providers / Bioss Polyclonal Antibodies / Anti-FDC-SP (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-1766R-A594

Price: 489 EUR

Get for 489 EUR on Gentaur.com

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Product details

Modification site

None

Gene ID

494224

Swiss Prot

Q5R1M6

Target Protein/Peptide

FDC-SP

Subcellular locations

Secreted

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Other name

Anti-FDC-SP Polyclonal

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Also known as

FDC-SP Polyclonal Antibody

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with FDC-SP

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from rat FDC-SP

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Synonyms

FDC; FDCs; Follicular dendritic cell; Follicular dendritic cell secreted protein; Follicular dendritic cell; Follicular dendritic cell secreted peptide; FDC secreted protein.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Follicular dendritic cells are cells that facilitate antigen recognition by B cells in follicles. This kind of dendritic cell is not bone marrow derived and is not a kind of blood cell. It is purely a resident of the follicles of secondary lymphoid organs. B cells form germinal centers around follicular dendritic cells in lymphoid organs. Dendritic cells form from monocytes, white blood cells which circulate in the body and, depending on the right signal, can turn into dendritic cells or macrophages. The monocytes in turn are formed from stem cells in the bone marrow.In normal tissue this antibody identifies dendritic cells and a proportion of B lymphocytes. Evidence in pathological tissue and functional studies suggests it binds to an epitope expressed by antigen presenting cells. Within the macrophage/dendritic cell populations the epitope seen by RFD1 is coexpressed with RFD7 by a subset of cells that exhibit suppressive activity on T cell stimulation.