Product details

Target Protein/Peptide

CD4

Modification site

None

Gene ID

24932

Swiss Prot

P05540

Subcellular locations

Cytoplasm

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Applications

FCM, IF(IHC-P)

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Other name

Anti-CD4 Polyclonal

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Also known as

CD4 Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Applications with corresponding dilutions

FCM(1:20-100), IF(IHC-P)(1:50-200)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with CD4

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from rat CD4

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Synonyms

p55; W3/25; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen; CD4

Background information

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.

Anti-CD4 (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-0647R-A594

Price: 489 EUR