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Providers / Bioss Polyclonal Antibodies / Anti-Streptavidin (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-0437R-A594

Price: 489 EUR

Get for 489 EUR on Gentaur.com

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Product details

Subcellular locations

N/A

Swiss Prot

N/A

Gene ID

N/A

Modification site

None

Cross reactive species

Others

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Target Protein/Peptide

Streptavidin

Cross Reactive Species details

Streptavidin

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Antigen Source

Recombinant Streptavidin

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Other name

Anti-Streptavidin Polyclonal

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

Streptavidin Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Modification

No modification has been applied to this antibody

Specificity

This antibody reacts specifically with Streptavidin

Synonyms

SA protein; SA V1; SA V2; Streptavidin V1; Streptavidin V2; SAV1_STRVL.

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.