Product details

Swiss Prot

N/A

Subcellular locations

N/A

Target Protein/Peptide

IL-3

Modification site

None

Gene ID

3562

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Cross reactive species

Human (Homo sapiens)

Other name

Anti-IL-3 Polyclonal

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Also known as

IL-3 Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with IL-3

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human IL-3

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Synonyms

Burst promoting activity; Colony stimulating factor multiple; Eosinophil colony stimulating factor; Hematopoietic growth factor; IL-3; IL3; IL3_HUMAN; Interleukin 3 colony stimulating factor, multiple; IL 3; Interleukin 3; Mast cell growth factor; MCGF; MGC79398; MGC79399; Multi CSF; Multilineage colony stimulating factor; Multipotential colony stimulating factor; P cell stimulating factor; Thy1 inducing factor; BPA; HCGF; Il 3; Interleukin-3; Mast cell growth factor; MCGF; Multi CSF; OTTHUMP00000065963; P-cell-stimulating factor; MCGF; MGC79399; Csfmu; Hematopoietic growth factor; IL3B; Interleukin 3 beta; Interleukin 3 precursor; Mast-cell growth factor; MGC79398; MULTI-CSF; Multilineage colony stimulating factor; Multipotential colony-stimulating factor; P cell stimulating factor; PSF; RP23-309E16.5.

Background information

The pleiotropic IL3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.

Anti-IL-3 (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-4510R-A594

Price: 489 EUR