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Providers / Bioss Polyclonal Antibodies / Anti-Histone H2A.X (Ser140), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-3185R-A594

Price: 516 EUR

Get for 516 EUR on Gentaur.com

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Product details

Gene ID

3014

Modification site

Ser140

Swiss Prot

P16104

Subcellular locations

Nucleus

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Modification

Phosphorylation

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Target Protein/Peptide

Histone H2A.X Ser140

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Type

Conjugated Primary Antibody

Other name

Anti-Histone H2A.X (Ser140)

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

Histone H2A.X (Ser140) Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Synonyms

H2AX; H2A.X; H2A/X; Histone H2AX; Histone H2A.X; H2AFX

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Specificity

This phosphorylation site is homologous to that of Ser140 in Mouse and Rat.Histone H2A.X (Ser140)

Antigen Source

KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.