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Providers / Bioss Polyclonal Antibodies / Anti-BrdU (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-0917R-A594

Price: 489 EUR

Get for 489 EUR on Gentaur.com

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Product details

Gene ID

N/A

Subcellular locations

N/A

Swiss Prot

N/A

Cross Reactive Species details

BrdU

Target Protein/Peptide

BrdU

Modification site

None

Cross reactive species

Others

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Other name

Anti-BrdU Polyclonal

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Antigen Source

BrdU conjugated to KLH

Also known as

BrdU Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with BrdU

Modification

No modification has been applied to this antibody

Synonyms

Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; proliferation Marker.

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Background information

Bromodeoxyuridine is a synthetic nucleoside that is an analogue of thymidine. BrdU is incorporated into DNA during S-phase of a cell cycle. It is commonly used in the detection of proliferating cells in living tissues

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.