Description:

Size: 100 microliters

Catalog no.: GENTObs-9300R-A594

Price: 489 EUR

Product details

Swiss Prot

N/A

Subcellular locations

N/A

Modification site

None

Gene ID

8568

Target Protein/Peptide

NNP1

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Other name

Anti-NNP1 Polyclonal

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Also known as

NNP1 Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with NNP1

Modification

No modification has been applied to this antibody

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Antigen Source

KLH conjugated synthetic peptide derived from human NNP1/RRP1 like protein

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Synonyms

NNP 1; NNP-1; NNP1; Nnp1 homolog nucleolar protein; Novel nuclear protein 1; Nucleolar protein Nop52; Protein NNP 1; Protein NNP1; Ribosomal RNA processing 1 homolog; Ribosomal RNA processing protein 1 homolog A; rrp1; RRP1 like protein; RRP1-like protein; RRP1_HUMAN; RRP1A.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Novel nuclear protein 1 (NNP-1), also known as RRP1-like protein or Nucleolar protein Nop52, is a 461 amino acid protein belonging to the RRP1 family. Localized to the nucleolus, NNP-1 has simian virus 40-type and bipartite nuclear localization signals and four coiled-coil domains within its C-terminal region. NNP-1 has been found to play an important role in the generation of 28S rRNA in the late processing steps of ribosome biogenesis. At the end of mitosis, nucleolar proteins assemble in a sequential order during the rebuilding of the nucleolus. NNP-1 assembles after Fibrillarin and C23, and simultaneously with B23 and POP1 in the prenucleolar body pathway.