0208080893 nl@gentaur.com
    Providers / Bioss Polyclonal Antibodies / Anti-HP1 alpha (Polyclonal), ALEXA Fluor 594

    Description:

    Size: 100 microliters

    Catalog no.: GENTObs-3825R-A594

    Price: 489 EUR

    Get for 489 EUR on Gentaur.com

    /ningbo-assets-bio/graphics/prods/bigs/bigs4.jpg

    Product details

    Subcellular locations

    N/A

    Swiss Prot

    N/A

    Modification site

    None

    Gene ID

    23468

    Target Protein/Peptide

    HP1 alpha

    Applications

    IF(IHC-P)

    Excitation emission

    590nm/617nm

    Concentration

    1ug per 1ul

    Conjugated

    Alexa conjugate 1

    Conjugated with

    ALEXA FLUOR® 594

    Applications with corresponding dilutions

    IF(IHC-P)(1:50-200)

    Clonality

    Polyclonal Antibody

    Clone

    Polyclonal Antibodies

    Purification method

    Purified by Protein A.

    Other name

    Anti-HP1 alpha Polyclonal

    Group

    Polyclonals and antibodies

    Type

    Conjugated Primary Antibody

    Also known as

    HP1 alpha Polyclonal Antibody

    Conjugation

    Alexa Fluor,ALEXA FLUOR® 594

    Host organism

    Rabbit (Oryctolagus cuniculus)

    Properties

    For facs or microscopy Alexa 1 conjugate.

    Specificity

    This antibody reacts specifically with HP1 alpha

    Modification

    No modification has been applied to this antibody

    Antigen Source

    KLH conjugated synthetic peptide derived from human HP1 alpha

    Cross reactive species

    Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

    Storage

    Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

    About

    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

    Cross Reactive Species details

    No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

    Description

    The Anti-HP1 alpha is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

    Synonyms

    Antigen p25; HP1-α; CBX5; CBX5_HUMAN; Chromobox homolog 5 HP1 alpha homolog, Drosophila; Chromobox homolog 5; Chromobox protein homolog 5; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1 homolog alpha; HP1 alpha; HP1 alpha homolog; HP1; HP1A; HP1Hs alpha.

    Advisory

    Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

    Background information

    HP1 alpha is a component of heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. HP1 alpha may interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, the chromo shadow domain. Both domains of HP1 are requiredfor binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin.