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Providers / Bioss Polyclonal Antibodies / Anti-PDHA1(Ser293) (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-4036R-A594

Price: 516 EUR

Get for 516 EUR on Gentaur.com

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Product details

Gene ID

5160

Swiss Prot

P08559

Modification site

Ser293

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Target Protein/Peptide

PDHA1 Ser293

Subcellular locations

Mitochondrion

Modification

Phosphorylation

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Other name

Anti-PDHA1(Ser293) Polyclonal

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

PDHA1(Ser293) Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Antigen Source

KLH conjugated synthetic phosphopeptide derived from human PDHA1 around the phosphorylation site of Ser293

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Synonyms

PDHA; PDHAD; PHE1A; PDHCE1A; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; PDHA1

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Background information

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Specificity

This phosphorylation site is homologous across the listed species. This antibody will detect PDHA2, the testis specific form, when phosphorylated at Ser293 in Human, Ser296 in Mouse, and the predicted site of Ser296 in Rat due to sequence homology.PDHA1(Ser293)

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.