0208080893 [email protected]
Providers / Bioss Polyclonal Antibodies / Anti-beta III Tubulin (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-4512R-A594

Price: 489 EUR

Get for 489 EUR on Gentaur.com

/ningbo-assets-bio/graphics/prods/bigs/bigs3.jpg

Product details

Modification site

None

Gene ID

10381

Swiss Prot

Q13509

Subcellular locations

Cytoplasm

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Target Protein/Peptide

beta III Tubulin

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications

IF(IHC-P), IF(ICC)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Other name

Anti-beta III Tubulin Polyclonal

Also known as

beta III Tubulin Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Properties

For facs or microscopy Alexa 1 conjugate.

Modification

No modification has been applied to this antibody

Specificity

This antibody reacts specifically with beta III Tubulin

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Antigen Source

KLH conjugated synthetic peptide derived from human Neuron specific beta III Tubulin

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Synonyms

CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; TUBB3

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Background information

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.