Description:

Size: 100 microliters

Catalog no.: GENTObs-2071R-A594

Price: 489 EUR

Product details

Swiss Prot

N/A

Gene ID

N/A

Subcellular locations

N/A

Modification site

None

Cross reactive species

Tags

Target Protein/Peptide

E2 tag

Cross Reactive Species details

E2 tag

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal Antibodies

Other name

Anti-E2 tag Polyclonal

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Also known as

E2 tag Polyclonal Antibody

Type

Conjugated Primary Antibody

Antigen Source

KLH conjugated E2 Tag peptide

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with E2 tag

Modification

No modification has been applied to this antibody

Synonyms

E2-tag; E2 epitope tag; SSTSSDFRDR epitope tag; SSTSSDFRDR tag.

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Background information

The E2 Tag is a peptide which is derived from the Bovine Pappillomavirus type-1 transactivator protein E2 and consists of 10 amino acids(SSTSSDFRDR). The detection of E2-tagged proteins is based on the binding of mouse monoclonal specific to the tagged recombinant protein.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.