Description:

Size: 100 microliters

Catalog no.: GENTObs-1174R-A594

Price: 489 EUR

Product details

Modification site

None

Gene ID

12578

Swiss Prot

P51480

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Target Protein/Peptide

CDKN2A/p19ARF

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Subcellular locations

Cytoplasm, Nucleus

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Other name

Anti-CDKN2A/p19ARF Polyclonal

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

CDKN2A/p19ARF Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Cross reactive species

Mouse (Mus musculus), Rat (Rattus norvegicus)

Modification

No modification has been applied to this antibody

Specificity

This antibody reacts specifically with CDKN2A/p19ARF

Antigen Source

KLH conjugated synthetic peptide derived from mouse CDKN2A/p19ARF

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Background information

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

Synonyms

Arf; p16; MTS1; Pctr1; p19ARF; p16INK4a; p19; ARF-INK4a; INK4a-ARF; Ink4a/Arf; p16(INK4a); Cyclin-dependent kinase inhibitor 2A, isoforms 1/2; Cyclin-dependent kinase 4 inhibitor A; CDK4I; p16-INK4a; p16-INK4; Cdkn2a

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.