Product details

Swiss Prot

N/A

Modification site

None

Gene ID

2827

Target Protein/Peptide

GPR3

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Subcellular locations

Extracellular

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Other name

Anti-GPR3 Polyclonal

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Also known as

GPR3 Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with GPR3

Modification

No modification has been applied to this antibody

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Antigen Source

KLH conjugated synthetic peptide derived from human G-protein coupled receptor 3

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Synonyms

ACCA; ACCA orphan receptor; Adenylate cyclase constitutive activator; G protein coupled receptor 3; G-protein coupled receptor 3; Gpr3; GPR3_HUMAN; Probable G protein coupled receptor GPR3; GPCR GPR3.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

G protein-coupled receptor 3 (GPR3), also designated ACCA orphan receptor, is a 330 amino acid member of the G protein-coupled receptor 1 family. The function of GPR3 is mediated by G proteins which activate adenylate cyclase. GPR3 is a mutli-pass membrane protein that is located on the cellular membrane of cells and is detected at low levels in the eye, kidney, lung, ovary and testis. GPR3 is most highly expressed in the central nervous system, where it stimulates the production of cAMP, leading to neurite outgrowth and myelin inhibition. In oocytes, this control over cAMP production can halt meiosis and prevent progesterone-induced meiotic maturation. Mice deficient for GPR3 are able to reproduce but have no control over the oocyte maturation process, which results in nondeveloping early embryos and fragmented oocytes as the mice age.

Anti-GPR3 (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-13529R-A594

Price: 489 EUR