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Providers / Bioss Polyclonal Antibodies / Anti-PKA R2 (Ser96) (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-4022R-A594

Price: 516 EUR

Get for 516 EUR on Gentaur.com

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Product details

Gene ID

5576

Modification site

Ser96

Swiss Prot

P13861

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Target Protein/Peptide

PKA R2 Ser96

Modification

Phosphorylation

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Subcellular locations

Cytoplasm, Cell membrane

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Other name

Anti-PKA R2 (Ser96) Polyclonal

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

PKA R2 (Ser96) Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Cross reactive species

Mouse (Mus musculus), Rat (Rattus norvegicus)

Specificity

This phosphorylation site is homologous to that of Ser97 in Rat.PKA R2 (Ser96)

Synonyms

PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit; PRKAR2A

Antigen Source

KLH conjugated synthetic phosphopeptide derived from mouse PKA R2 around the phosphorylation site of Ser96

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Background information

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.