Description:

    Size: 100 microliters

    Catalog no.: GENTObs-13184R-A594

    Price: 516 EUR

    Product details

    Swiss Prot

    N/A

    Subcellular locations

    N/A

    Gene ID

    2322

    Modification site

    Tyr599

    Applications

    IF(IHC-P)

    Concentration

    1ug per 1ul

    Target Protein/Peptide

    FLT3 Tyr599

    Excitation emission

    590nm/617nm

    Modification

    Phosphorylation

    Conjugated with

    ALEXA FLUOR® 594

    Conjugated

    Alexa conjugate 1

    Clonality

    Polyclonal Antibody

    Applications with corresponding dilutions

    IF(IHC-P)(1:50-200)

    Clone

    Polyclonal Antibodies

    Purification method

    Purified by Protein A.

    Group

    Polyclonals and antibodies

    Type

    Conjugated Primary Antibody

    Other name

    Anti-FLT3 (Tyr599) Polyclonal

    Conjugation

    Alexa Fluor,ALEXA FLUOR® 594

    Host organism

    Rabbit (Oryctolagus cuniculus)

    Also known as

    FLT3 (Tyr599) Polyclonal Antibody

    Properties

    For facs or microscopy Alexa 1 conjugate.

    Specificity

    This antibody reacts specifically with FLT3 (Tyr599)

    Cross reactive species

    Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

    Antigen Source

    KLH conjugated synthetic phosphopeptide derived from human FLT3 around the phosphorylation site of Tyr599

    Storage

    Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

    Description

    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

    Synonyms

    Receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; Fetal liver kinase-2; FLK-2; Fms-like tyrosine kinase 3; FLT-3; Stem cell tyrosine kinase 1; STK-1; FLT3; CD135; FLK2; STK1

    About

    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

    Cross Reactive Species details

    No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

    Advisory

    Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

    Background information

    Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.