Product details

Subcellular locations

N/A

Swiss Prot

N/A

Modification site

None

Gene ID

66037

Target Protein/Peptide

BOULE

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Other name

Anti-BOULE Polyclonal

Purification method

Purified by Protein A.

Also known as

BOULE Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with BOULE

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human BOULE

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Synonyms

Bol Drosophila boule homolog like; Bol; Bol boule like; Bol, boule like Drosophila; bol, boule-like Drosophila; Boll; BOLL_HUMAN; BOULE; Boule like; BOULE, Drosophila, homolog of; Protein boule like; Protein boule-like; Putative uncharacterized protein BOLL.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Spermatogenesis represents the intricate developmental process of mitotic and meiotic cell divisions that ultimately leads to the production of haploid spermatozoa. BOULE, a member of the human deleted in azoospermia (DAZ) family, functions as a key conserved switch that regulates the progression of germ cells through meiosis in man. BOULE is an RNA-binding protein that regulates the expression of twine, a Cdc25 phosphatase, which promotes progression through meiosis. BOULE is expressed not only in the testis, but also in the nervous system, where it may play a role in neural communication. Mutations in the BOULE gene are be associated with male infertility, and the relative proportions of the three BOULE isoforms (B1, B2 and B3) may function as predictive markers for meiotic efficiency.

Anti-BOULE (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-12880R-A594

Price: 489 EUR