Description:

Size: 100ul

Catalog no.: bs-1370R-A488

Price: 380 EUR

Product details

Target Antigen

ATM

Modification Site

None

Gene ID Number

11920

Swiss Prot

Q62388

French translation

anticorps

Clonality

Polyclonal

Modification

Unmodified

Excitation emission

499nm/519nm

Concentration

1ug per 1ul

Conjugation

Alexa Fluor

Immunogen range

2700-2750/3066

Crossreactivity

Human, Mouse, Rat

Conjugated with

ALEXA FLUOR® 488

Tested applications

IF(IHC-P), IF(ICC)

Subcellular location

Cytoplasm, Nucleus

Clone

Polyclonal antibody

Purification

Purified by Protein A.

Also known as

Anti-ATM PAb ALEXA FLUOR 488

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Recommended dilutions

IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Specificity

This is a highly specific antibody against ATM.

Long name

ATM Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

Source

KLH conjugated synthetic peptide derived from mouse ATM

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Synonyms

AI256621; C030026E19Rik; Serine-protein kinase ATM; Ataxia telangiectasia mutated homolog; A-T mutated homolog; Atm

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-ATM has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Binds DNA ends. Plays a role in replication-dependent histone mRNA degradation. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response.