Description:

Size: 100ul

Catalog no.: bs-0349R-A488

Price: 380 EUR

Product details

Modification Site

None

Gene ID Number

3020

Swiss Prot

P84243

Subcellular location

Nucleus

French translation

anticorps

Clonality

Polyclonal

Immunogen range

90-136/136

Target Antigen

Histone H3

Modification

Unmodified

Conjugation

Alexa Fluor

Excitation emission

499nm/519nm

Concentration

1ug per 1ul

Tested applications

FCM, IF(IHC-P)

Conjugated with

ALEXA FLUOR® 488

Clone

Polyclonal antibody

Crossreactivity

Human, Mouse, Rat, Pig

Purification

Purified by Protein A.

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Recommended dilutions

FCM(1:20-100), IF(IHC-P)(1:50-200)

Also known as

Anti-Histone H3 PAb ALEXA FLUOR 488

Synonyms

H3F3; H3.3A; Histone H3.3; H3F3A; PP781

Specificity

This is a highly specific antibody against Histone H3.

Long name

Histone H3 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Histone H3

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Histone H3 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.