Description:

    Size: 100ul

    Catalog no.: bs-3686R-A350

    Price: 380 EUR

    Product details

    Modification Site

    None

    Target Antigen

    PHD2

    Gene ID Number

    54583

    Swiss Prot

    Q9GZT9

    French translation

    anticorps

    Immunogen range

    30-70/426

    Tested applications

    IF(IHC-P)

    Clonality

    Polyclonal

    Modification

    Unmodified

    Excitation emission

    343nm/442nm

    Concentration

    1ug per 1ul

    Crossreactivity

    Human, Mouse

    Conjugated with

    ALEXA FLUOR® 350

    Subcellular location

    Cytoplasm, Nucleus

    Clone

    Polyclonal antibody

    Recommended dilutions

    IF(IHC-P)(1:50-200)

    Purification

    Purified by Protein A.

    Conjugation

    Alexa Fluor,ALEXA FLUOR 350

    Also known as

    Anti-PHD2 PAb ALEXA FLUOR 350

    Category

    Conjugated Primary Antibodies

    Host Organism

    Rabbit (Oryctolagus cuniculus)

    Specificity

    This is a highly specific antibody against PHD2.

    Long name

    PHD2 Polyclonal Antibody, ALEXA FLUOR 350 Conjugated

    Cross-reactive species details

    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

    Source

    This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human PHD2

    Storage conditions

    Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

    Synonyms

    HPH2; PHD2; SM20; ECYT3; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2; Egl nine homolog 1; Hypoxia-inducible factor prolyl hydroxylase 2; HIF-prolyl hydroxylase 2; Prolyl hydroxylase domain-containing protein 2; SM-20; EGLN1; PNAS-118; PNAS-137

    Properties

    For facs or microscopy Alexa 1 conjugate.Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

    Background of the antigen

    Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferencially recognized via a LXXLAP motif.