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    Providers / Bioss Primary Conjugated Antibodies. ALEXA FLUOR / Smad3 (Ser213) Antibody, ALEXA FLUOR 488

    Description:

    Size: 100ul

    Catalog no.: bs-5459R-A488

    Price: 350 EUR

    Get for 350 EUR on Gentaur.com

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    Product details

    Gene ID Number

    4088

    Modification Site

    Ser213

    Tested applications

    IF(IHC-P)

    French translation

    anticorps

    Clonality

    Polyclonal

    Conjugation

    Alexa Fluor

    Excitation emission

    499nm/519nm

    Concentration

    1ug per 1ul

    Target Antigen

    Smad3 Ser213

    Modification

    Phosphorylation

    Crossreactivity

    Human, Mouse, Rat

    Conjugated with

    ALEXA FLUOR® 488

    Recommended dilutions

    IF(IHC-P)(1:50-200)

    Clone

    Polyclonal antibody

    Purification

    Purified by Protein A.

    Category

    Conjugated Primary Antibodies

    Host Organism

    Rabbit (Oryctolagus cuniculus)

    Also known as

    Anti-Smad3 Ser213 PAb ALEXA FLUOR 488

    Specificity

    This is a highly specific antibody against Smad3 Ser213.

    Long name

    Smad3 (Ser213) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

    Cross-reactive species details

    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

    Source

    KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser213 [PM(p-S)PA]

    Storage conditions

    Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

    Synonyms

    SMAD3phospho S213; SMAD3phospho Ser213; p-SMAD3Ser213; hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD mothers against decapentaplegic Drosophila homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD-3; SMAD3_HUMAN.

    Properties

    For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Smad3 Ser213 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

    Background of the antigen

    Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.