Description:

Size: 100ul

Catalog no.: bs-3045R-A594

Price: 350 EUR

Product details

Gene ID Number

51789

Modification Site

Tyr284

Swiss Prot

O54967

French translation

anticorps

Clonality

Polyclonal

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Target Antigen

Ack1 Tyr284

Immunogen range

260-310/1055

Tested applications

FCM, IF(IHC-P)

Modification

Phosphorylation

Conjugated with

ALEXA FLUOR® 594

Crossreactivity

Human, Mouse, Rat

Conjugated

Alexa conjugate 1

Clone

Polyclonal antibody

Purification

Purified by Protein A.

Subcellular location

Nucleus, Membrane bound

Category

Conjugated Primary Antibodies

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host Organism

Rabbit (Oryctolagus cuniculus)

Recommended dilutions

FCM(1:20-100), IF(IHC-P)(1:50-200)

Also known as

Anti-Ack1 Tyr284 PAb ALEXA FLUOR 594

Specificity

This is a highly specific antibody against Ack1 Tyr284.

Long name

Ack1 (Tyr284) Polyclonal Antibody, ALEXA FLUOR 594 Conjugated

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from mouse Ack1 around the phosphorylation site of Tyr284

Synonyms

Ack; Ack1; Pyk1; Ack-1; Cdgip; Activated CDC42 kinase 1; Non-receptor protein tyrosine kinase Ack; Tyrosine kinase non-receptor protein 2; Tnk2

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Properties

For facs or microscopy Alexa 1 conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.