Description:

    Size: 100ul

    Catalog no.: bs-2777R-A555

    Price: 380 EUR

    Product details

    Gene ID Number

    5925

    Modification Site

    None

    Swiss Prot

    P06400

    Subcellular location

    Nucleus

    Target Antigen

    P105 RB

    French translation

    anticorps

    Tested applications

    IF(IHC-P)

    Clonality

    Polyclonal

    Immunogen range

    68-116/928

    Modification

    Unmodified

    Excitation emission

    553nm/568nm

    Concentration

    1ug per 1ul

    Conjugated with

    ALEXA FLUOR® 555

    Crossreactivity

    Human, Mouse, Rat

    Clone

    Polyclonal antibody

    Recommended dilutions

    IF(IHC-P)(1:50-200)

    Purification

    Purified by Protein A.

    Conjugation

    Alexa Fluor,ALEXA FLUOR 555

    Category

    Conjugated Primary Antibodies

    Host Organism

    Rabbit (Oryctolagus cuniculus)

    Also known as

    Anti-P105 RB PAb ALEXA FLUOR 555

    Specificity

    This is a highly specific antibody against P105 RB.

    Long name

    P105 RB Polyclonal Antibody, ALEXA FLUOR 555 Conjugated

    Synonyms

    RB; pRb; OSRC; pp11; p15-Rb; PPP1R13; Retinoblastoma-associated protein; RB1

    Cross-reactive species details

    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

    Source

    This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Rb/P105 RB

    Storage conditions

    Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

    Properties

    For facs or microscopy Alexa 1 conjugate.Very high photo stable ALEXA conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

    Background of the antigen

    Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV42H1 and SUV42H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-2' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV4 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.