Product details

Clone

1F6

Modification Site

None

Gene ID Number

7124

Crossreactivity

Human

Swiss Prot

P01375

Target Antigen

TNF-alpha

Tested applications

IF(IHC-P)

French translation

anticorps

Modification

Unmodified

Clonality

Monoclonal

Concentration

1ug per 1ul

Conjugation

Alexa Fluor

Excitation emission

499nm/519nm

Conjugated with

ALEXA FLUOR® 488

Recommended dilutions

IF(IHC-P)(1:50-200)

Host Organism

Mouse (Mus musculus)

Purification

Purified by Protein G.

Subcellular location

Secreted, Membrane bound

Also known as

Anti-TNF-alpha MAb ALEXA FLUOR 488

Specificity

This is a highly specific antibody against TNF-alpha.

Long name

TNF-alpha (1F6) Monoclonal Antibody, ALEXA FLUOR 488 Conjugated

Source

This antibody is produced using full length recombinant human TNF-alpha.

Description

The Anti-TNF-alpha is a α- or alpha protein sometimes glycoprotein present in blood.

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Synonyms

DIF; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; Cachectin; Tumor necrosis factor ligand superfamily member 2; TNF-a; TNF

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Background of the antigen

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Gene

Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-TNF-alpha has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

TNF-alpha (1F6) Monoclonal Antibody, ALEXA FLUOR 4 ...

Description:

Size: 100ul

Catalog no.: bsm-0387M-A488

Price: 380 EUR