Description:

Size: 100ul

Catalog no.: bs-5243R-A488

Price: 350 EUR

Product details

Gene ID Number

994

Swiss Prot

P30305

Modification Site

Ser323

French translation

anticorps

Subcellular location

Cytoplasm

Tested applications

IF(IHC-P)

Clonality

Polyclonal

Conjugation

Alexa Fluor

Concentration

1ug per 1ul

Excitation emission

499nm/519nm

Immunogen range

300-350/580

Target Antigen

Cdc25B Ser323

Modification

Phosphorylation

Conjugated with

ALEXA FLUOR® 488

Crossreactivity

Human, Mouse, Rat

Clone

Polyclonal antibody

Recommended dilutions

IF(IHC-P)(1:50-200)

Purification

Purified by Protein A.

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-Cdc25B Ser323 PAb ALEXA FLUOR 488

Specificity

This is a highly specific antibody against Cdc25B Ser323.

Long name

Cdc25B (Ser323) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

Synonyms

M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; CDC25B; CDC25HU2

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser323

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Background of the antigen

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Cdc25B Ser323 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.