Description:

Size: 100ul

Catalog no.: bs-3575R-A488

Price: 380 EUR

Product details

Modification Site

None

Target Antigen

SSEA3

French translation

anticorps

Modification

Unmodified

Clonality

Polyclonal

Crossreactivity

Human, Rat

Conjugation

Alexa Fluor

Excitation emission

499nm/519nm

Concentration

1ug per 1ul

Subcellular location

Cell membrane

Conjugated with

ALEXA FLUOR® 488

Tested applications

IF(IHC-P), IF(ICC)

Clone

Polyclonal antibody

Purification

Purified by Protein A.

Source

4-8 cell stage mouse embryos

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-SSEA3 PAb ALEXA FLUOR 488

Recommended dilutions

IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Specificity

This is a highly specific antibody against SSEA3.

Long name

SSEA3 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

Synonyms

SSEA-3; Stage specic embryonic antigen 3; Stage specic embryonic antigen 3.

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-SSEA3 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

SSEA3 is a globoseries carbohydrate antigen present on both cell surface glycolipids and glycopeptides. It is found on the surface of mouse oocytes, becoming restricted to the intracellular mass of the early blastocyst and then to the primitive endoderm. It is present on the surface of human teratocarcinoma cells (EC), embryonic germ cells (EG) and embryonic stem cells (ES) but not mouse undifferentiated mouse cells of these types. SSEA3 expression decreases as human EC, ES and EG cells differentiate but increases on differentiation of mouse EC, ES and EG cells. It is also found on the surface of mouse erythrocytes.