Description:

Size: 100ul

Catalog no.: bs-0737R-A488

Price: 380 EUR

Product details

Gene ID Number

3091

Modification Site

None

Swiss Prot

Q16665

French translation

anticorps

Modification

Unmodified

Clonality

Polyclonal

Excitation emission

499nm/519nm

Concentration

1ug per 1ul

Immunogen range

351-400/826

Target Antigen

HIF-1 Alpha

Conjugation

Alexa Fluor

Conjugated with

ALEXA FLUOR® 488

Crossreactivity

Human, Mouse, Rat

Tested applications

IF(IHC-P), IF(ICC)

Subcellular location

Cytoplasm, Nucleus

Clone

Polyclonal antibody

Purification

Purified by Protein A.

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-HIF-1 Alpha PAb ALEXA FLUOR 488

Recommended dilutions

IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Specificity

This is a highly specific antibody against HIF-1 Alpha.

Long name

HIF-1 Alpha Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

Description

The Anti-HIF-1 Alpha is a α- or alpha protein sometimes glycoprotein present in blood.

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human of human HIF-1 Alpha

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Synonyms

HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; Member of PAS protein 1; PAS domain-containing protein 8; HIF1A

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-HIF-1 Alpha has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 4 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP3. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.