Description:

Size: 100ul

Catalog no.: bs-12569R-A488

Price: 350 EUR

Product details

Gene ID Number

6521

Modification Site

Tyr21

Crossreactivity

Human

Tested applications

IF(IHC-P)

French translation

anticorps

Clonality

Polyclonal

Target Antigen

Band3 Tyr21

Concentration

1ug per 1ul

Excitation emission

499nm/519nm

Conjugation

Alexa Fluor

Modification

Phosphorylation

Conjugated with

ALEXA FLUOR® 488

Clone

Polyclonal antibody

Recommended dilutions

IF(IHC-P)(1:50-200)

Purification

Purified by Protein A.

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-Band3 Tyr21 PAb ALEXA FLUOR 488

Specificity

This is a highly specific antibody against Band3 Tyr21.

Long name

Band3 (Tyr21) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human Band3 around the phosphorylation site of Tyr21

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Synonyms

Band 3 phospho Y21; p-Band 3 phospho Y21; Solute carrier family 4 anion exchanger member 1; Solute carrier family 4 member 1; AE 1; AE1; Anion exchange protein 1; Anion exchanger 1; B3AT_HUMAN; Band 3; Band 3 anion transport protein; BND3; CD233; DI; Diego blood group; EMPB3; EPB3; Erythrocyte membrane protein band 3; Erythroid anion exchange protein; FR antibod; Froese blood group; RTA1A; SLC4A1; Solute carrier family 4 member 1; SW antibody; Swann blood group; Waldner blood group; WD antibody; WD1; WR antibody; Wright blood group.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Band3 Tyr21 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

Band 3, also designated AE1, is an erythrocyte membrane glycoprotein that contributes to cell stuctural integrity and mediates exchange of chloride and bicarbonate across the phospholipid bilayer. The diverse functions of the approximately 900 amino acid protein are mediated by two distinct domains. The amino terminal domain, also known as cdb3 for cytoplasmic domain of erthrocyte membrane band 3, acts as an attachment site for the erythrocyte skeleton by binding ankyrin. The carboxy-terminal, membrane-associated domain carries out exchange transport of anions. Degradation of band 3 can generate an aging antigen known as senescent cell antigen, or SCA, which is expressed on old cells and marks them for removal by the immune system. An isoform of band 3, which lacks the first 65 amino acids and does not bind ankryin, is expressed in kidney.