Description:

    Size: 100ul

    Catalog no.: bs-0545R-A488

    Price: 380 EUR

    Product details

    Target Antigen

    SCF

    Modification Site

    None

    Gene ID Number

    4254

    Swiss Prot

    P21583

    French translation

    anticorps

    Clonality

    Polyclonal

    Modification

    Unmodified

    Conjugation

    Alexa Fluor

    Immunogen range

    160-210/273

    Concentration

    1ug per 1ul

    Excitation emission

    499nm/519nm

    Subcellular location

    Extracellular

    Conjugated with

    ALEXA FLUOR® 488

    Crossreactivity

    Human, Mouse, Rat

    Clone

    Polyclonal antibody

    Purification

    Purified by Protein A.

    Tested applications

    FCM, IF(IHC-P), IF(IHC-F)

    Also known as

    Anti-SCF PAb ALEXA FLUOR 488

    Category

    Conjugated Primary Antibodies

    Host Organism

    Rabbit (Oryctolagus cuniculus)

    Specificity

    This is a highly specific antibody against SCF.

    Long name

    SCF Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

    Recommended dilutions

    FCM(1:20-100), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200)

    Cross-reactive species details

    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

    Synonyms

    SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7; Kit ligand; Mast cell growth factor; Stem cell factor; c-Kit ligand; KITLG

    Source

    This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human SCF

    Storage conditions

    Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

    Properties

    For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-SCF has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

    Background of the antigen

    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.