Description:

Size: 100ul

Catalog no.: bs-11255R-A555

Price: 380 EUR

Product details

Target Antigen

NSF

Modification Site

None

French translation

anticorps

Tested applications

IF(IHC-P)

Clonality

Polyclonal

Modification

Unmodified

Excitation emission

553nm/568nm

Concentration

1ug per 1ul

Conjugated with

ALEXA FLUOR® 555

Crossreactivity

Human, Mouse, Rat

Recommended dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal antibody

Purification

Purified by Protein A.

Conjugation

Alexa Fluor,ALEXA FLUOR 555

Also known as

Anti-NSF PAb ALEXA FLUOR 555

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Specificity

This is a highly specific antibody against NSF.

Long name

NSF Polyclonal Antibody, ALEXA FLUOR 555 Conjugated

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human NSF

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Properties

For facs or microscopy Alexa 1 conjugate.Very high photo stable ALEXA conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Synonyms

N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor like protein; N ethylmaleimide sensitive fusion protein; N ethylmaleimide sensitive fusion protein; N-ethylmaleimide-sensitive fusion protein; NEM sensitive fusion protein; NEM sensitive fusion protein; NEM-sensitive fusion protein; NSF; NSF_HUMAN; SKD 2; SKD2; SKD2; Vesicle fusing ATPase; Vesicle fusing ATPase; Vesicle-fusing ATPase; Vesicular fusion protein NSF; Vesicular fusion protein NSF; Vesicular-fusion protein NSF.

Background of the antigen

Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).