Description:

Size: 100ul

Catalog no.: bs-2327R-A488

Price: 380 EUR

Product details

Virus

hbv

Modification Site

None

Crossreactivity

Virus

French translation

anticorps

Tested applications

IF(IHC-P)

Modification

Unmodified

Clonality

Polyclonal

Excitation emission

499nm/519nm

Conjugation

Alexa Fluor

Concentration

1ug per 1ul

Conjugated with

ALEXA FLUOR® 488

Recommended dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal antibody

Target Antigen

HBV pre S1/S2 protein

Purification

Purified by Protein A.

Cross-reactive species details

Hepatitis B virus ay/ad

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-HBV pre S1/S2 protein PAb ALEXA FLUOR 488

Long name

HBV pre S1/S2 protein Antibody, ALEXA FLUOR 488 Conjugated

Specificity

This is a highly specific antibody against HBV pre S1/S2 protein.

Source

KLH conjugated synthetic peptide derived from HBSAG_HBVD2 sub. ayw pre S1/S2 protein

Synonyms

HBSAG_HBVD2; HBSAG_HBVC5; Large envelope protein; Large surface protein; L glycoprotein; Major surface antigen; Large S protein; L-HBsAg; LHB.

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-HBV pre S1/S2 protein has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.