Description:

Size: 100ul

Catalog no.: bs-0081R-A647

Price: 380 EUR

Product details

Gene ID Number

836

Modification Site

None

Swiss Prot

P42574

Target Antigen

Caspase-3

Subcellular location

Cytoplasm

French translation

anticorps

Immunogen range

60-100/277

Modification

Unmodified

Clonality

Polyclonal

Excitation emission

650nm/665nm

Concentration

1ug per 1ul

Conjugated with

ALEXA FLUOR® 647

Clone

Polyclonal antibody

Purification

Purified by Protein A.

Tested applications

FCM, IF(IHC-P), IF(ICC)

Conjugation

Alexa Fluor,ALEXA FLUOR 647

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Crossreactivity

Human, Mouse, Rat, Bovine, Goat

Also known as

Anti-Caspase-3 PAb ALEXA FLUOR 647

Specificity

This is a highly specific antibody against Caspase-3.

Recommended dilutions

FCM(1:20-100), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Long name

Caspase 3 Polyclonal Antibody, ALEXA FLUOR 647 Conjugated

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Caspase 3(Active)

Synonyms

CPP32; SCA-1; CPP32B; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; CASP3

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

Background of the antigen

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-Caspase-3 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.