Product details

Gene ID Number

26986

Swiss Prot

P11940

Tested applications

IF(IHC-P)

French translation

anticorps

Clonality

Polyclonal

Concentration

1ug per 1ul

Immunogen range

440-480/636

Modification

Methylation

Conjugation

Alexa Fluor

Excitation emission

499nm/519nm

Crossreactivity

Human, Mouse, Rat

Modification Site

Methyl Arg455/460

Conjugated with

ALEXA FLUOR® 488

Subcellular location

Cytoplasm, Nucleus

Recommended dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal antibody

Target Antigen

PABP Methyl Arg455/460

Purification

Purified by Protein A.

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-PABP Methyl Arg455/460 PAb ALEXA FLUOR 488

Long name

PABP (Methyl Arg455/460) Antibody, ALEXA FLUOR 488 Conjugated

Specificity

This is a highly specific antibody against PABP Methyl Arg455/460.

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic metylpeptide derived from human PABP around metylation site of Arg455/Arg460

Synonyms

PAB1; PABP; PABP1; PABPC2; PABPL1; Polyadenylate-binding protein 1; PABP-1; Poly(A)-binding protein 1; PABPC1

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Description

A methyl group is an alkyl derived from methane, containing one carbon atom bonded to three hydrogen atoms — CH3. In formulas, the group is often abbreviated Me. Such hydrocarbon groups occur in many organic compounds. It is a very stable group in most molecules. While the methyl group is usually part of a larger molecule, it can be found on its own in any of three forms anion, cation or radical. The anion has eight valence electrons, the radical seven and the cation six. All three forms are highly reactive and rarely observed,

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-PABP Methyl Arg455/460 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

Binds the poly(A) tail of mRNA, including that of its own transcript. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed.

PABP (Methyl Arg455/460) Antibody, ALEXA FLUOR 488

Description:

Size: 100ul

Catalog no.: bs-3836R-A488

Price: 350 EUR