Description:

Size: 100ul

Catalog no.: bs-0052R-A594

Price: 380 EUR

Product details

Modification Site

None

Gene ID Number

12370

Swiss Prot

O89110

Target Antigen

Caspase 8

Subcellular location

Cytoplasm

French translation

anticorps

Modification

Unmodified

Clonality

Polyclonal

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Immunogen range

430-482/482

Crossreactivity

Human, Mouse, Rat

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Tested applications

IF(IHC-P), IF(ICC)

Clone

Polyclonal antibody

Purification

Purified by Protein A.

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-Caspase 8 PAb ALEXA FLUOR 594

Recommended dilutions

IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Synonyms

MACH; Mch5; FLICE; CASP-8; Caspase-8; Casp8

Specificity

This is a highly specific antibody against Caspase 8.

Long name

Caspase 8 Polyclonal Antibody, ALEXA FLUOR 594 Conjugated

Source

KLH conjugated synthetic peptide derived from mouse Caspase-8 subunit p10

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Properties

For facs or microscopy Alexa 1 conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

Background of the antigen

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP1. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.