Description:

Size: 100ul

Catalog no.: bs-0542R-A555

Price: 380 EUR

Product details

Modification Site

None

Target Antigen

CDK1

Gene ID Number

12534

Swiss Prot

P11440

French translation

anticorps

Clonality

Polyclonal

Modification

Unmodified

Excitation emission

553nm/568nm

Immunogen range

250-297/297

Concentration

1ug per 1ul

Tested applications

FCM, IF(IHC-P)

Crossreactivity

Human, Mouse, Rat

Conjugated with

ALEXA FLUOR® 555

Clone

Polyclonal antibody

Purification

Purified by Protein A.

Conjugation

Alexa Fluor,ALEXA FLUOR 555

Category

Conjugated Primary Antibodies

Also known as

Anti-CDK1 PAb ALEXA FLUOR 555

Host Organism

Rabbit (Oryctolagus cuniculus)

Subcellular location

Cytoplasm, Nucleus, Mitochondrion

Recommended dilutions

FCM(1:20-100), IF(IHC-P)(1:50-200)

Specificity

This is a highly specific antibody against CDK1.

Long name

CDK1 Polyclonal Antibody, ALEXA FLUOR 555 Conjugated

Source

KLH conjugated synthetic peptide derived from mouse CDK1

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Synonyms

Cdc2; Cdc2a; p34; Cyclin-dependent kinase 1; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; Cdkn1

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Properties

For facs or microscopy Alexa 1 conjugate.Very high photo stable ALEXA conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

The cell division control protein cdc2, also known as cyclin dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.